A test for the dihydroxyacetone phosphate pathway
نویسندگان
چکیده
منابع مشابه
Glycerolipid biosynthesis in peroxisomes via the acyl dihydroxyacetone phosphate pathway.
In recent years, acyl dihydroxyacetone phosphate [acyl-DHAPt) has been shown to be a precursor of glycerolipids and glycerol-ether lipids.'.' Acyl-DHAP was discovered as a rapidly labeled lipid that was formed in crude mitochondrial fraction from 3'Pi or y-32P[ATP].3 This rapid labeling was due to the enzymatic dephosphorylation and rephosphorylation of endogenous acyl-DHAP present in the crude...
متن کاملThe acyl dihydroxyacetone phosphate pathway enzymes for glycerolipid biosynthesis are present in the yeast Saccharomyces cerevisiae.
The presence of the acyl dihydroxyacetone phosphate (acyl DHAP) pathway in yeasts was investigated by examining three key enzyme activities of this pathway in Saccharomyces cerevisiae. In the total membrane fraction of S. cerevisiae, we confirmed the presence of both DHAP acyltransferase (DHAPAT; Km = 1.27 mM; Vmax = 5.9 nmol/min/mg of protein) and sn-glycerol 3-phosphate acyltransferase (GPAT;...
متن کاملThe acyl dihydroxyacetone phosphate pathway for glycerolipid biosynthesis in mouse liver and Ehrlich ascites tumor cells.
The glycerol portion of lipids may be derived biosynthetically by reduction of dihydroxyacetone phosphate to glycerolphosphate, and then be acylated with fatty acids or, alternatively, dihydroxyacetone phosphate may first be acylated and then reduced to 1-acyl sn glcerol-3-phosphate. Since the former pathway utilizes NADH for reduction of the C-2 carbonyl, while the latter requires NADPH, we we...
متن کاملExclusive localization in peroxisomes of dihydroxyacetone phosphate acyltransferase and alkyl-dihydroxyacetone phosphate synthase in rat liver.
Dihydroxyacetone phosphate acyl transferase (DHAP-AT), alkyl dihydroxyacetone phosphate synthase (alkyl-DHAP-synthase), and glycerol-3-phosphate acyltransferase (GPAT) activities were investigated under optimal assay conditions using highly purified organelle preparations. The data presented clearly indicate that GPAT activity was mainly localized in mitochondria and microsomes, whereas DHAP-AT...
متن کاملNovel listerial glycerol dehydrogenase- and phosphoenolpyruvate-dependent dihydroxyacetone kinase system connected to the pentose phosphate pathway.
Several bacteria use glycerol dehydrogenase to transform glycerol into dihydroxyacetone (Dha). Dha is subsequently converted into Dha phosphate (Dha-P) by an ATP- or phosphoenolpyruvate (PEP)-dependent Dha kinase. Listeria innocua possesses two potential PEP-dependent Dha kinases. One is encoded by 3 of the 11 genes forming the glycerol (gol) operon. This operon also contains golD (lin0362), wh...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
سال: 1970
ISSN: 0005-2760
DOI: 10.1016/0005-2760(70)90160-8